Evaluation of Satb1, survivin and Ki-67 as possible markers to differentiate phyllodes tumor from fibroadenoma
Aims: Difficulties in diagnosis of phyllodes tumour are well known. Our aim was to observe whether the expressions of satb1, survivin and ki-67 were helpful in differentiating fibroadenoma and phyllodes tumour. Settings and Design: Retrospective Methods and Material: Paraffin embedded tissue samples from 60 female patients with phyllodes tumour & 60 female patients with fibroadenoma were studied. Expression of the gene products was studied and confirmed using Immunohistochemical and western blot analysis Statistical analysis used: The statistical analysis was performed using Fisher’s Exact test to find out the significant changes Results: Statistically significant difference was observed in Satb1, survivin expression between fibroadenoma and benign phyllodes cases. The difference between the Ki-67 expression in fibroadenoma and benign phylloodes cases was not statistically significant. Conclusions: Our finding of strong stromal expression of satb1 and survivin in phyllodes as compared to fibroadenoma can be helpful for the development of additional diagnostic and prognostic indicators for otherwise difficult cases. Such immunochemical markers can be used to elucidate cellular basis of tumor behaviour. Validation of such immunochemical test in future will reduce diagnostic uncertainty in this rare tumor. In addition to that such parameters may serve as a therapeutic target that could increase effectiveness of chemotherapy or radiation therapy. A study with large number of samples along with clinical and follow-up data is required for confirmation.Key-words: Phyllodes, Satb1, Survivin, Ki-67,fibroadenoma
A S Kapali, M Singh, SVS Deo, N K Shukla, and Dillip K Muduly. Aggressive Palliative Surgery in Metastatic Phyllodes Tumor: Impact on Quality of Life. Indian J Palliat Care. 2010 ; 16(2): 101–104.
R K Jacklin, P F Ridgway, P Ziprin, V Healy, D Hadjiminas, and A Darzi. Optimising preoperative diagnosis in phyllodes tumour of the breast. J Clin Pathol. 2006; 59(5): 454–459.
Ranjana Bandyopadhyay, Dipanwita Nag, Santosh Kumar Mondal, Subhalakshmi Mukhopadhyay, Sumit Roy, and Swapan Kumar Sinha. Distinction of phyllodes tumor from fibroadenoma: Cytologists’ perspective. J Cytol. 2010 ; 27(2): 59–62.
Neill Patani, Wen Jiang, Robert Mansel, Robert Newbold and Kefah Mokbel. The mRNA expression of SATB1 and SATB2 in human breast cancer Cancer Cell International 2009;9:18
Han HJ, Russo J, Kohwi Y, Kohwi-Shigematsu T. SATB1 reprogrammes gene expression to promote breast tumour growth and metastasis.Nature. 2008;452:187-93.
Zheng J. Is SATB1 a master regulator in breast cancer growth and metastasis?.Womens Health (Lond Engl). 2008;4:329-32.
Kim YA, Chang M, Park YJ, Kim JE.Detection of Survivin and COX-2 in Thyroid Carcinoma: Anaplastic Carcinoma Shows Overexpression of Nuclear Survivin and Low COX-2 Expression. Korean J Pathol. 2012;46(1):55-60.
Ambrosini G,Adida C and Altieri D : A novel anti apoptosis gene,survivin expressed in cancer and lymphoma.Nat Med 3;1997:917-921
Thompson CB.nesis and treatment of disease.Science 1995;267:1456-1462.
Marzia Pennati, Marco Folini and Nadia Zaffaroni. Targeting survivin in cancer therapy: fulfilled promises and open questions. Carcinogenesis. 2007;vol.28 no.6 pp.1133–1139,
Miglietta L, Vanella P, Canobbio L, Naso C, Cerisola N, Meszaros P, et al. Ki-67 index after neoadjuvant chemotherapy in locally advanced breast cancerexpressing high levels of proliferation at diagnosis. Oncology. 2010;79:255-61.
Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown.J Cell Physiol. 2000;182:311-22.
Yohe S, Yeh IT."Missed" diagnoses of phyllodes tumor on breast biopsy: pathologic clues to its recognition. Int J Surg Pathol. 2008;16:137-42
Kang Y, Kim JH, Lee TH, Kim TS, Jung WH, Chung HC, et al. Expression of anaphase-promoting complex7 in fibroadenomas and phyllodes tumors of breast. Hum Pathol. 2009;40:98-107.
Jacklin RK, Ridgway PF, Ziprin P, Healy V, Hadjiminas D, Darzi A. Optimising preoperative diagnosis in phyllodes tumour of the breast. J Clin Pathol. 2006 ;59:454-9.
Li FY, Wu SG, Zhou J, Sun JY, Lin Q, Lin HX, Guan XX, He ZY. Prognostic value of Ki-67 in breast cancer patients with positive axillary lymph nodes: a retrospective cohort study.PLoS One. 2014;9(2):e87264.
Cattoretti G,Suurmeijer AJH:Antigen unmasking on formalin fixed paraffin embedded tissue using microwave:a review.Advances in anatomy and pathology. 1995;2:2-9
Tahrin Mahmood and Ping-Chang Yang. Western Blot: Technique, Theory, and Trouble Shooting. N Am J Med Sci. 2012; 4(9): 429–434.
Shan-rong Shi, cheng liu, Brian M. Balgley, cheng lee, clive R. et al. Protein extraction from formalin-fixed,paraffin-embedded tissue sections: Quality evaluation by mass spectrometry. Histochem Cytochem.2006;54:739-743.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
Marcella Macaluso, Marco G Paggi and Antonio Giordano. Genetic and epigenetic alterations as hallmarks of the intricate road to cancer. Oncogene (2003) 22, 6472–6478.
Mir R, Pradhan SJ, Galande S. Chromatin organizer SATB1 as a novel molecular target for cancer therapy. Curr Drug Targets. 2012 Sep 17. [Epub ahead of print]
Fuqiang Sun,,* Xiaoming Lu, Hang Li Zhao Peng, Ke Wu, Guobin Wang, And Qiang Tong. Special AT-rich sequence binding protein 1 regulates the multidrug resistance and invasion of human gastric cancer cells. Oncol Lett. 2012; 4(1): 156–162.
Alltieri DC,Marchisio C.Survivin apoptosis; an interloper between cell death and cell proliferation in cancer.Lab Invest 1999;79:13233
Nassar A, Lawson D, Cotsonis G, Cohen C. Survivin and caspase-3 expression in breast cancer: correlation with prognostic parameters, proliferation, angiogenesis, and outcome. Appl Immunohistochem Mol Morphol. 2008;16(2):113-20
Ana Richelia Jara-Lazaro and Puay Hoon Tan. Molecular pathogenesis of progression and recurrence in breast phyllodes tumors. Am J Transl Res. 2009; 1(1): 23–34.
Sawhney N, Garrahan N, Douglas-Jones AG, Williams ED.Epithelial--stromal interactions in tumors. A morphologic study of fibroepithelial tumors of the breast. Cancer. 1992;70(8):2115-20.
Jian Song, Hong Su, Yang-yang Zhou, Liang-liang Guo. Prognostic value of survivin expression in breast cancer patients: a meta-analysis . TUMOR BIOLOGY. 2013, Volume 34, Issue 4, pp 2053–2062
Ribeiro-Silva A, Zambelli Ramalho LN, Zucoloto S. Phyllodes tumor with osteosarcomatous differentiation: a comparative immunohistochemical study between epithelial and mesenchymal cells.Tumori.2006;92:340-6.
E. C. Inwald M. Klinkhammer-Schalke, F. Hofstädter, F. Zeman, M. Koller, M. Gerstenhauer et al. Ki-67 is a prognostic parameter in breast cancer patients: results of a large population-based cohort of a cancer registry.Breast Cancer Res Treat. 2013; 139(2): 539–552.
Jara-Lazaro AR, Akhilesh M, Thike AA, Lui PC, Tse GM, Tan PH. Predictors of phyllodes tumours on core biopsy specimens of fibroepithelial neoplasms. Histopathology.2010;57:220-32.
Kleer CG, Giordano TJ, Braun T, Oberman HA. Pathologic, immunohistochemical, and molecular features of benign and malignant phyllodes tumors of the breast. Mod Pathol. 2001 ;14(3):185-90.
Lee KH, Koh M, Moon A. Farnesyl transferase inhibitor FTI-277 inhibits breast cell invasion and migration by blocking H-Ras activation. Oncol Lett. 2016;12(3):2222-2226.
Alshareeda AT2, Negm OH, Aleskandarany MA, Green AR, Nolan C, TigHhe PJ. Clinical and biological significance of RAD51 expression in breast cancer: a key DNA damage response protein.Breast Cancer Res Treat. 2016;159(1):41-53.
Gallardo M, Calaf GM. Curcumin inhibits invasive capabilities through epithelial mesenchymal transition in breast cancer cell lines.Int J Oncol. 2016;49(3):1019-27.
Tawasil J, Go EM, Tsang JY, Ni YB, Ko CW, Tse GM. Associations of epithelial c-kit expression in phyllodes tumours of the breast.J Clin Pathol. 2015;68(10):808-11.
This work is licensed under a Creative Commons Attribution 3.0 License.
Print ISSN: 2347-6192
Online ISSN: 2347-6206