Prevalence of Methicillin Resistant Staphylococcus aureus, Extended Spectrum of β Lactamase Producing Escherichia coli and Klebsiella Spps Isolates from ICU Patients at Tertiary Care Hospital, Aurangabad
Background and Objectives: The microbial surveillance of ICUs for multidrug resistant bugs is required for management of ICU patients. The objectives of the study were to find out the prevalence and antimicrobial susceptibility pattern of MRSA and ESBLs producing E.coli and Klebsiella isolates in clinical samples from ICU patients. Methods: A total of 464 clinical samples received in the department of microbiology for culture and sensitivity from ICU patients and were processed as per standard protocol. Detection of MRSA and ESBLs were detected by using CLSI guidelines. Results: 164 were positive for culture. A total of 196 isolates were isolated, among that 57 were S. aureus and 34 were E.coli, 51 were Klebsiella spp and remaining 54 isolates were other gram negative and gram positive organisms. Out of 57 S. aureus 23 (42.6%) were detected as MRSA. 41.2% of E. coli and 45.1% of Klebsiella spp were ESBL producers. The antibiotic sensitivity rates were found higher in MRSA than MSSA and also same in non-ESBL-producing and ESBL-producing strains which were statistically significant (p-value). Conclusion: The maximum ESBL producing isolates were Klebsiella spp than E.coli. Vancomycin and linezolid remains a drug of choice for MRSA. For ESBL producing E.coli and Klebsiella, carbapenems remain the drug of choice. Institutional antimicrobial surveillance and proper infection control practices are essential to prevent and control multi drug resistant bugs in ICUs and hospital.Key Words: ICU, ESBLs, MRSA, VANCOMYCIN, IMIPENAM
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